Open AccessPlasma-derived exosome-like vesicles are enriched in lyso-phospholipids and pass the blood-brain barrier
Author(s) -
Martin Jakubec,
Jodi MapleGrødem,
Saleha Akbari,
Susanne Nesse,
Øyvind Halskau
Publication year - 2020
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0232442
Subject(s) - microvesicles , vesicle , exosome , blood–brain barrier , liposome , phosphatidylserine , microbiology and biotechnology , lipid bilayer , chemistry , biophysics , biology , membrane , biochemistry , phospholipid , central nervous system , microrna , neuroscience , gene
Exosomes are vesicles involved in intercellular communication. Their membrane structure and core content is largely dependent on the cell of origin. Exosomes have been investigated both for their biological roles and their possible use as disease biomarkers and drug carriers. These potential technological applications require the rigorous characterization of exosomal blood brain barrier permeability and a description of their lipid bilayer composition. To achieve these goals, we have established a 3D static blood brain barrier system based on existing systems for liposomes and a complementary LC-MS/MS and 31 P nuclear magnetic resonance methodology for the analysis of purified human plasma-derived exosome-like vesicles. Results show that the isolated vesicles pass the blood brain barrier and are taken up in endothelial cells. The compositional analysis revealed that the isolated vesicles are enriched in lyso phospholipids and do not contain phosphatidylserine. These findings deviate significantly from the composition of exosomes originating from cell culture, and may reflect active removal by macrophages that respond to exposed phosphahtidylserine.