Open AccessCharacterization of a novel compound that promotes myogenesis via Akt and transcriptional co-activator with PDZ-binding motif (TAZ) in mouse C2C12 cells
Author(s) -
Manami Kodaka,
Fengju Mao,
Kyoko ArimotoMatsuzaki,
Masami Kitamura,
Xiaoyin Xu,
Zeyu Yang,
Kentaro Nakagawa,
Junichi Maruyama,
Kayoko Ishii,
Chihiro Akazawa,
Takuya Oyaizu,
Naoki Yamamoto,
Mari IshigamiYuasa,
Nozomi Tsuemoto,
Shigeru Ito,
Hiroyuki Kagechika,
Hiroshi Nishina,
Yoshinobu Hata
Publication year - 2020
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0231265
Subject(s) - myogenesis , c2c12 , microbiology and biotechnology , activator (genetics) , phosphorylation , biology , protein kinase b , chemistry , myocyte , biochemistry , receptor
Transcriptional co-activator with PDZ-binding motif (TAZ) plays versatile roles in the regulation of cell proliferation and differentiation. TAZ activity changes in response to the cellular environment such as mechanic and nutritional stimuli, osmolarity, and hypoxia. To understand the physiological roles of TAZ, chemical compounds that activate TAZ in cells are useful as experimental reagents. Kaempferol, TM-25659, and ethacridine are reported as TAZ activators. However, as each TAZ activator has a distinct property in cellular functions, additional TAZ activators are awaiting. We screened for TAZ activators and previously reported IB008738 as a TAZ activator that promotes myogenesis in C2C12 cells. In this study, we have characterized IBS004735 that was obtained in the same screening. IBS004735 also promotes myogenesis in C2C12 cells, but is not similar to IBS008738 in the structure. IBS004735 activates TAZ via Akt and has no effect on TAZ phosphorylation, which is the well-described key modification to regulate TAZ activity. Thus, we introduce IBS004735 as a novel TAZ activator that regulates TAZ in a yet unidentified mechanism.