Open AccessMultifamily QTL analysis and comprehensive design of genotypes for high-quality soft wheat
Author(s) -
Goro Ishikawa,
Takeshi Hayashi,
Kazuhiro Nakamura,
Tsuyoshi Tanaka,
Fuminori Kobayashi,
Mika Sillanpää,
Hiroyuki Ito,
Sachiko Ikenaga,
Yoshinori Taniguchi,
Toshiki Nakamura
Publication year - 2020
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0230326
Subject(s) - quantitative trait locus , biology , heritability , doubled haploidy , family based qtl mapping , marker assisted selection , selection (genetic algorithm) , genetics , allele , locus (genetics) , trait , microbiology and biotechnology , gene mapping , chromosome , gene , artificial intelligence , computer science , programming language
Milling properties and flour color are essential selection criteria in soft wheat breeding. However, high phenotypic screening costs restrict selection to relatively few breeding lines in late generations. To achieve marker-based selection of these traits in early generations, we performed genetic dissection of quality traits using three doubled haploid populations that shared the high-quality soft wheat variety Kitahonami as the paternal parent. An amplicon sequencing approach allowed effective construction of well-saturated linkage maps of the populations. Marker-based heritability estimates revealed that target quality traits had relatively high values, indicating the possibility of selection in early generations. Taking advantage of Chinese Spring reference sequences, joint linkage maps of the three populations were generated. Based on the maps, multifamily quantitative trait locus (QTL) analysis revealed a total of 86 QTLs for ten traits investigated. In terms of target quality traits, 12 QTLs were detected for flour yield, and 12 were detected for flour redness (a* value). Among these QTLs, six for flour yield and nine for flour a* were segregating in more than two populations. Some relationships among traits were explained by QTL collocations on chromosomes, especially group 7 chromosomes. Ten different ideotypes with various combinations of favorable alleles for the flour yield and flour a* QTLs were generated. Phenotypes of derivatives from these ideotypes were predicted to design ideal genotypes for high-quality wheat. Simulations revealed the possibility of breeding varieties with better quality than Kitahonami.