Open AccessExpression of lamin C2 in mammalian oocytes
Author(s) -
Markéta Končická,
Jakub Červenka,
Daniel Jahn,
Rita Suchá,
Petr Vodička,
Ahmed Gad,
Manfred Alsheimer,
Andrej Šušor
Publication year - 2020
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0229781
Subject(s) - germinal vesicle , lamin , biology , oocyte , microbiology and biotechnology , gene expression , meiosis , nuclear lamina , gene , embryo , genetics , nuclear protein , nucleus , transcription factor
Lamin C2 (LMN C2) is a short product of the lamin a gene. It is a germ cell-specific lamin and has been extensively studied in male germ cells. In this study, we focussed on the expression and localization of LMN C2 in fully-grown germinal vesicle (GV) oocytes. We detected LMN C2 in the fully-grown germinal vesicle oocytes of various mammalian species with confirmation done by immunoblotting the wild type and Lmnc2 gene deleted testes. Expression of LMN C2 tagged with GFP showed localization of LMN C2 to the nuclear membrane of the oocyte. Moreover, the LMN C2 protein notably disappeared after nuclear envelope breakdown (NEBD) and the expression of LMN C2 was significantly reduced in the oocytes from aged females and ceased altogether during meiotic maturation. These results provide new insights regarding LMN C2 expression in the oocytes of various mammalian species.