Open AccessDiffusion MRI reveals in vivo and non-invasively changes in astrocyte function induced by an aquaporin-4 inhibitor
Author(s) -
Clement Debaker,
Boucif Djemaï,
Luisa Ciobanu,
Tomokazu Tsurugizawa,
Denis Le Bihan
Publication year - 2020
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0229702
Subject(s) - aquaporin 4 , astrocyte , in vivo , glymphatic system , aquaporin , hippocampus , effective diffusion coefficient , chemistry , diffusion , water transport , neuroscience , biophysics , pathology , medicine , central nervous system , biology , magnetic resonance imaging , biochemistry , physics , water flow , cerebrospinal fluid , thermodynamics , microbiology and biotechnology , radiology , environmental engineering , engineering
The Glymphatic System (GS) has been proposed as a mechanism to clear brain tissue from waste. Its dysfunction might lead to several brain pathologies, including the Alzheimer’s disease. A key component of the GS and brain tissue water circulation is the astrocyte which is regulated by acquaporin-4 (AQP4), a membrane-bound water channel on the astrocytic end-feet. Here we investigated the potential of diffusion MRI to monitor astrocyte activity in a mouse brain model through the inhibition of AQP4 channels with TGN-020. Upon TGN-020 injection, we observed a significant decrease in the Sindex, a diffusion marker of tissue microstructure, and a significant increase of the water diffusion coefficient (sADC) in cerebral cortex and hippocampus compared to saline injection. These results indicate the suitability of diffusion MRI to monitor astrocytic activity in vivo and non-invasively.