Open AccessA new synthetic medium for the optimization of docosahexaenoic acid production in Crypthecodinium cohnii
Author(s) -
Pingping Song,
Alexander Kuryatov,
Paul H. Axelsen
Publication year - 2020
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0229556
Subject(s) - docosahexaenoic acid , yeast extract , heterotroph , food science , biology , yeast , mixotroph , algae , polyunsaturated fatty acid , biochemistry , botany , fatty acid , bacteria , fermentation , genetics
The heterotrophic microalgae Crypthecodinium cohnii was usually cultivated in complex medium containing glucose, yeast extract and sea salt. For the preparation of DHA with highest purity, a new defined medium without the yeast extract was developed. Different inoculated densities, C/N ratios, temperatures, culture volumes and glucose additions were investigated to optimize the algal growth rate and DHA production. The growth period in C . cohnii was shortened from 12–14 days to 7–8 days, the OD 600 was enhanced from 2.0 to 3.0, the glucose consumption was accelerated and used up on day 3–4, and the DHA content in culture were increased from 10 to 45 nmoles/300 μl batch. It was found that C . cohnii had optimal growth and DHA accumulation in 25 °C, 0.2 inoculated density, 5–10 C/N ratio, 5:1 air/culture volume ratio. This is the first time DHA production using C . cohnii has been optimized in synthetic medium. This allows preparation of uniformly radiolabeled 13 C- and 14 C-DHA.