Open AccessGenotoxic effect of 2,2’-bis(bicyclo[2.2.1] heptane) on bacterial cells
Author(s) -
A. Kessenikh,
E. Yu. Gnuchikh,
Sergey V. Bazhenov,
М. В. Бермешев,
В. Г. Певгов,
Vadim O. Samoilov,
Sergey V. Shorunov,
А. Л. Максимов,
Lev S. Yaguzhinsky,
И. В. Манухов
Publication year - 2020
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0228525
Subject(s) - regulon , sos response , escherichia coli , genotoxicity , reactive oxygen species , dna damage , heptane , superoxide , oxidative stress , chemistry , dna , pro oxidant , luciferase , promoter , biochemistry , biology , microbiology and biotechnology , gene , gene expression , toxicity , enzyme , transfection , organic chemistry
The toxic effect of strained hydrocarbon 2,2'—bis (bicyclo[2.2.1]heptane) (BBH) was studied using whole-cell bacterial lux-biosensors based on Escherichia coli cells in which luciferase genes are transcriptionally fused with stress-inducible promoters. It was shown that BBH has the genotoxic effect causing bacterial SOS response however no alkylating effect has been revealed. In addition to DNA damage, there is an oxidative effect causing the response of OxyR/S and SoxR/S regulons. The most sensitive to BBH lux-biosensor was E . coli pSoxS-lux which reacts to the appearance of superoxide anion radicals in the cell. It is assumed that the oxidation of BBH leads to the generation of reactive oxygen species, which provide the main contribution to the genotoxicity of this substance.