Open AccessExpression of the muscle-associated gene MYF6 in hairy cell leukemia
Author(s) -
Evgeny Arons,
Hong Zhou,
Mark Sokolsky,
Daniel Gorelik,
Katherine Potocka,
Sarah Davies,
Erin Fykes,
Katherine Still,
Daniel C. Edelman,
Yonghong Wang,
Paul S. Meltzer,
Mark Raffeld,
Adrian Wiestner,
Liqiang Xi,
Haowei Wang,
Maryalice StetlerStevenson,
Constance Yuan,
Robert J. Kreitman
Publication year - 2020
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0227586
Subject(s) - hairy cell leukemia , biology , interleukin 3 receptor , chronic lymphocytic leukemia , leukemia , microbiology and biotechnology , flow cytometry , immunology
Hairy cell leukemia (HCL) is a purine analog-responsive B-cell malignancy containing the BRAF V600E mutation, expressing CD22, CD11c, CD103, tartrate resistant acid phosphatase (TRAP) CD25, CD123, and annexin 1A. BRAF V600E and the latter 4 markers are usually absent in the more aggressive and chemoresistant variant HCLv. To evaluate differences between HCL and HCLv, expression microarrays comparing HCL with HCLv were performed for 24694 genes using 47323 probes. Microarray data from 35 HCL and 27 HCLv purified samples showed the greatest HCL-HCLv difference in the muscle-associated gene MYF6 , expressed by its 2 probes 18.5- and 10.8-fold higher in HCL than HCLv (p<0.0001). By real-time quantitative PCR (RQ-PCR), 100% of 152 classic HCL samples were MYF6 -positive, vs 5 (6%) of 90 blood donors. MYF6 -expression was also detected in 18 (35%) of 51 with HCLv, 11 (92%) of 12 with HCL expressing unmutated IGHV4-34, 35 (73%) of 48 with chronic lymphocytic leukemia (CLL), and 1 (8%) of 12 with mantle cell lymphoma. Hypomethylation status of MYF6 supported expression in HCL more than HCLv. Posttreatment blood samples becoming negative by flow cytometry remained MYF6 + by RQ-PCR in 42 (48%) of 87 HCL patients, and MYF6 RQ-PCR could detect 1 HCL in 10 5 normal cells. MYF6 , universally expressed in HCL and in most CLL samples, may be a useful biomarker for these leukemias. Further studies are underway to determine the role of MYF6 in HCL.