Open AccessCentromeres of Cucumis melo L. comprise Cmcent and two novel repeats, CmSat162 and CmSat189
Author(s) -
Agus Budi Setiawan,
Chee How Teo,
Shinichi Kikuchi,
Hidenori Sassa,
Kenji Kato,
Takato Koba
Publication year - 2020
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0227578
Subject(s) - centromere , biology , cucumis , melon , subtelomere , genetics , chromosome , satellite dna , fluorescence in situ hybridization , genome , metaphase , meiosis , karyotype , gene , botany , horticulture
Centromeres are prerequisite for accurate segregation and are landmarks of primary constrictions of metaphase chromosomes in eukaryotes. In melon, high-copy-number satellite DNAs (SatDNAs) were found at various chromosomal locations such as centromeric, pericentromeric, and subtelomeric regions. In the present study, utilizing the published draft genome sequence of melon, two new SatDNAs ( CmSat162 and CmSat189 ) of melon were identified and their chromosomal distributions were confirmed using fluorescence in situ hybridization. DNA probes prepared from these SatDNAs were successfully hybridized to melon somatic and meiotic chromosomes. CmSat162 was located on 12 pairs of melon chromosomes and co-localized with the centromeric repeat, Cmcent , at the centromeric regions. In contrast, CmSat189 was found to be located not only on centromeric regions but also on specific regions of the chromosomes, allowing the characterization of individual chromosomes of melon. It was also shown that these SatDNAs were transcribed in melon. These results suggest that CmSat162 and CmSat189 might have some functions at the centromeric regions.