Open AccessAlleleProfileR: A versatile tool to identify and profile sequence variants in edited genomes
Author(s) -
Arne A.N. Bruyneel,
Alexandre R. Colas,
Ioannis Karakikes,
Mark Mercola
Publication year - 2019
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0226694
Subject(s) - transcription activator like effector nuclease , crispr , genome editing , zinc finger nuclease , palindrome , cas9 , computational biology , biology , genome , genetics , effector , gene , microbiology and biotechnology
Gene editing strategies, such as zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeat/Cas9 (CRISPR/Cas9), are revolutionizing biology. However, quantitative and sensitive detection of targeted mutations are required to evaluate and quantify the genome editing outcomes. Here we present AlleleProfileR, a new analysis tool, written in a combination of R and C++, with the ability to batch process the sequence analysis of large and complex genome editing experiments, including the recently developed base editing technologies.