Open AccessDense carbon-nanotube coating scaffolds stimulate osteogenic differentiation of mesenchymal stem cells
Author(s) -
Hideki Mori,
Yutaka Ogura,
Kenta Enomoto,
Masayuki Hara,
Gjertrud Maurstad,
Bjørn Torger Stokke,
Shinichi Kitamura
Publication year - 2020
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0225589
Subject(s) - carbon nanotube , mesenchymal stem cell , runx2 , materials science , osteocalcin , alkaline phosphatase , coating , scaffold , bone morphogenetic protein 2 , biomedical engineering , nanotechnology , chemistry , microbiology and biotechnology , biochemistry , in vitro , enzyme , medicine , biology
Carbon nanotubes (CNTs) have desirable mechanical properties for use as biomaterials in orthopedic and dental area such as bone- and tooth- substitutes. Here, we demonstrate that a glass surface densely coated with single-walled carbon nanotubes (SWNTs) stimulate the osteogenic differentiation of rat bone marrow mesenchymal stem cells (MSCs). MSCs incubated on SWNT- and multi-walled carbon nanotube (MWNT)-coated glass showed high activities of alkaline phosphatase that are markers for early stage osteogenic differentiation. Expression of Bmp2 , Runx2 , and Alpl of MSCs showed high level in the early stage for MSC incubation on SWNT- and MWNT-coated surfaces, but only the cells on the SWNT-coated glass showed high expression levels of Bglap ( Osteocalcin ). The cells on the SWNT-coated glass also contained the most calcium, and their calcium deposits had long needle-shaped crystals. SWNT coating at high density could be part of a new scaffold for bone regeneration.