Open AccessLiquid Chromatography/Mass Spectrometry based serum metabolomics study on recurrent abortion women with antiphospholipid syndrome
Author(s) -
Lili Zhang,
Ying Li,
Xiuying Lin,
Jia Chen,
Xiaowei Yu
Publication year - 2019
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0225463
Subject(s) - metabolomics , medicine , abortion , antiphospholipid syndrome , pregnancy , disease , products of conception , fetus , purine metabolism , bioinformatics , mechanism (biology) , metabolic pathway , multivariate analysis , physiology , metabolism , biology , biochemistry , thrombosis , genetics , enzyme , philosophy , epistemology
Objective The antiphospholipid syndrome (APS) is an important cause of acquired thromboembolic complications and pregnancy morbidity. The pathogenic mechanisms that damage the fetal–maternal unit and cause abnormal placental development are incompletely understood in APS patients. Liquid Chromatography/Mass Spectrometry (LC/MS) based metabolomics are applied for the mechanism of disease and further supporting the research of diagnosis and management in recent years. The aim of this research was to investigate the difference of serum metabolic profiles in recurrent abortion women with APS and healthy women to explore the mechanism of this disease. Methods Serum samples of 25 recurrent abortion women with APS and 25 healthy women were collected and analyzed by LC/MS in this study. Potential biomarkers were discovered by multivariate statistical analysis and then identified based on analysis results. Results Totally, we identified five biomarkers that involved in different metabolic pathway such as purine metabolism, amino acid metabolism and tyrosine metabolism. These biomarkers showed different roles in disease development. Conclusion Metabolomics was proved as a powerful tool in understanding the mechanism of recurrent abortion caused by APS.