Open AccessOccurrence and multilocus genotyping of Giardia duodenalis from post-weaned dairy calves in Sichuan province, China
Author(s) -
Jiaming Dan,
Xueping Zhang,
Zhihua Ren,
Liqin Wang,
Suizhong Cao,
Liuhong Shen,
Junliang Deng,
Zhuang Zuo,
Shumin Yu,
Ya Wang,
Xiaoping Ma,
Haifeng Liu,
Ziyao Zhou,
Yanchun Hu,
Hualin Fu,
Chao He,
Yi Geng,
Xiaobin Gu,
Guangneng Peng,
Zhijun Zhong
Publication year - 2019
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0224627
Subject(s) - biology , multilocus sequence typing , giardia , giardia lamblia , phylogenetic tree , feces , genotyping , genetic diversity , veterinary medicine , genotype , zoology , gene , genetics , ecology , population , medicine , demography , sociology
Giardia duodenalis is a zoonotic parasitic protist and poses a threat to human and animal health. This study investigated the occurrence of G . duodenalis infection in post-weaned calves from Sichuan province, China. Faecal samples were collected from a total of 306 post-weaned calves (3–12 months old) from 10 farms, including 4 intensive feeding farms and 6 free-ranging farms. The overall infection rate of G . duodenalis was 41.2% (126/306) based on the PCR results at any of the three genetic loci: beta-giardin ( bg ), triose-phosphate isomerase ( tpi ) and glutamate dehydrogenase ( gdh ) genes. Giardia duodenalis assemblages E (n = 115, 91.3%), A (n = 3, 2.4%), and A mixed with E (n = 8, 6.3%) were identified among the 126 positive specimens. Multilocus sequence typing of G . duodenalis revealed 34 assemblage E multilocus genotypes (MLGs), 1 assemblage A MLG and 7 mixed assemblage (A and E) MLGs. The eBURST data showed a high degree of genetic diversity within assemblage E MLGs. The phylogenetic tree revealed that MLG E3 was the primary MLG subtype in Sichuan province and also the most widely distributed in China.