Open AccessCombined immunization with attenuated live influenza vaccine and chimeric pneumococcal recombinant protein improves the outcome of virus-bacterial infection in mice
Author(s) -
Tatiana Kramskaya,
Galina Leontieva,
Yulia Desheva,
K. B. Grabovskaya,
Tatiana Gupalova,
Larisa Rudenko,
Alexander Suvorov
Publication year - 2019
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0222148
Subject(s) - virology , vaccination , streptococcus pneumoniae , immunization , microbiology and biotechnology , influenza a virus , virus , attenuated vaccine , pneumococcal infections , flagellin , biology , recombinant dna , influenza vaccine , serotype , virulence , medicine , immunology , immune system , bacteria , gene , antibiotics , genetics , biochemistry
Influenza and its bacterial complications are a leading cause of morbidity and mortality worldwide. The effect of combined immunization with live influenza vaccine and recombinant chimeric pneumococcal protein in dual infection caused by influenza H1N1 and S . pneumoniae (serotype 3) has been studied. The combined vaccine consisted of the strain A/California/2009/38 (H1N1) pdm and chimeric recombinant protein PSPF composed of immunodominant fragments of the surface virulence factors of S . pneumoniae— PsaA, PspA, and Shr1875—associated with modified salmonella flagellin. Vaccinated mice were infected with the influenza virus 24 hours before or 24 hours after the onset of pneumococcal infection. The protective effect of combined vaccination was shown on both models of viral-bacterial infection.