Open AccessRelationship between rumen ciliate protozoa and biohydrogenation fatty acid profile in rumen and meat of lambs
Author(s) -
Alexandra Francisco,
José SantosSilva,
Ana Paula Portugal,
Susana P. Alves,
Rui J.B. Bessa
Publication year - 2019
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0221996
Subject(s) - rumen , biology , ciliate , zoology , protozoa , fatty acid , food science , botany , biochemistry , ecology , fermentation
This study investigated the associations between abundance of rumen ciliate protozoa and the proportion of the main bioactive fatty acids related to rumen biohydrogenation, as 18:0, t 10-18:1, t 11-18:1, c 9, t 11-18:2, 18:3n-3 and 18:2 n-6, in rumen and meat of growing lambs, using data derived from 3 production experiments. A global correlation analysis and a linear regression analysis considering the effect of the experiment were performed. Ten of the 86 lambs involved in the experiments did not present ciliate cells in rumen liquor and the remaining lambs presented an average of 1.35 × 10 6 ciliates / ml rumen liquor. From the nine genera of ciliates identified, Entodinium was the most abundant, averaging 1.17 × 10 6 cells / ml of rumen liquor. A large variation among lambs was observed for both rumen concentration and community structure of ciliates. Rumen t 11-18:1 ( P < 0.001) and meat deposition of t 11-18:1 ( P < 0.001) and of c 9, t 11-18:2 ( P < 0.001) increased linearly with total ciliates, whereas the t 10/ t 11 ratio in rumen ( P = 0.002) and in meat ( P = 0.036) decreased linearly. Entodiniomorphids seems to be strongly related with meat deposition of t 11-18:1 and c 9, t 11-18:2 and with the reduction of the trans -10 shifted pathway. Completeness of RBH decreased linearly with Holotrichs ( P = 0.029), Entodiniomorphids ( P = 0.029), Isotricha ( P = 0.011) and Epidinium ( P = 0.027) abundances. Rumen 18:0 also decreased linearly with increasing counts of total ciliates ( P = 0.015), Holotrichs ( P = 0.020), Entodiniomorphids ( P = 0.010) and Isotricha ( P = 0.014). Rumen protozoa were positively linked with the deposition of healthy bioactive FA and simultaneously negatively associated with the occurrence of trans -10 shift.