Open AccessEstablishment of immortalized primary cell from the critically endangered Bonin flying fox (Pteropus pselaphon)
Author(s) -
Tohru Tani,
Takahiro Eitsuka,
Masafumi Katayama,
Takashi Nakagawa,
Yumiko Nakaya,
Hajime Suzuki,
Tohru Kiyono,
Kiyotaka Nakagawa,
Miho InoueMurayama,
Manabu Onuma,
Tomokazu Fukuda
Publication year - 2019
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0221364
Subject(s) - biology , telomerase , somatic cell , critically endangered , telomerase reverse transcriptase , somatic cell nuclear transfer , embryo , cyclin d1 , telomere , cell culture , endangered species , microbiology and biotechnology , cell , genetics , cell cycle , gene , blastocyst , embryogenesis , ecology , habitat
The Bonin flying fox ( Pteropus pselaphon ) is one of the most critically endangered species of animals. The number of this species is estimated to be around 150; being classified at the top rank in the list by International Union of Animal Conservation. Our group previously showed that expression of CDK4, CYCLIN D1, and telomerase reverse transcriptase (TERT) efficiently induce immortalization of human, bovine, swine, monkey, and buffalo-derived cells. In this manuscript, we successfully established the primary cells from Bonin flying fox. We introduced CDK4, CYCLIN D1, and TERT into the primary cells. The established cells showed efficient expression of introduced genes at the protein level. Furthermore, the established cells were free from senescence, indicating it reached to immortalization. Moreover, we showed that interspecies somatic cell nuclear transfer of Bonin flying fox derived cell into bovine embryo allowed the development of the embryo to 8 cell stages. Our established cell has the potential to contribute to species conservation.