Open AccessBeneficial roles of probiotics on the modulation of gut microbiota and immune response in pigs
Author(s) -
Donghyun Shin,
Sung-Ho Chang,
Paul Bogere,
KyeongHye Won,
Jae-Ho Choi,
YunSang Choi,
Hak Kyo Lee,
Jin Hur,
ByungYong Park,
Young-Hoon Kim,
Jae-Young Heo
Publication year - 2019
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0220843
Subject(s) - biology , gut flora , probiotic , microbiology and biotechnology , immune system , lactobacillus plantarum , metagenomics , innate immune system , transcriptome , lactobacillus , population , firmicutes , butyrate , microbiome , immunology , bacteria , gene expression , gene , genetics , lactic acid , food science , fermentation , 16s ribosomal rna , demography , sociology
The importance of probiotics in swine production is widely acknowledged as crucial. However, gaps still remain in the exact roles played by probiotics in modulation of gut microbiota and immune response. This study determined the roles of probiotic Lactobacillus plantarum strain JDFM LP11in gut microbiota modulation and immune response in weaned piglets. L . plantarum JDFM LP11 increased the population of lactic acid bacteria in feces and enhanced the development of villi in the small intestine. Metagenome analysis showed that microbial diversity and richness (Simpson, Shannon, ACE, Chao1) and the relative abundance of the Firmicutes were higher in weaned piglets fed probiotics. Five bacterial families were different in the relative abundance, especially; Prevotellaceae occupied the largest part of microbial community showed the most difference between two groups. Transcriptome analysis identified 25 differentially expressed genes using RNA-sequencing data of the ileum. Further gene ontology and immune DB analysis determined 8 genes associated with innate defense response and cytokine production. BPI, RSAD2, SLPI, LUM, OLFM4, DMBT1 and C6 genes were down-regulated by probiotic supplementation except PLA2G2A. PICRUSt analysis predicting functional profiling of microbial communities indicated branched amino acid biosynthesis and butyrate metabolism promoting gut development and health were increased by probiotics. Altogether, our data suggest that L . plantarum JDFM LP11 increases the diversity and richness in the microbial community, and attenuates the ileal immune gene expression towards gut inflammation, promoting intestinal development in weaned piglets.