Open AccessCoagulant activity of recombinant human factor VII produced by lentiviral human F7 gene transfer in immortalized hepatocyte-like cell line
Author(s) -
Sarai Pongjantarasatian,
Praguywan Kadegasem,
Werasak Sasanakul,
Khanit Sangiamsuntorn,
Suparerk Borwornpinyo,
gnuch Sirachainan,
Ampaiwan Chuansumrit,
Pansakorn Tanratana,
Suradej Hongeng
Publication year - 2019
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0220825
Subject(s) - cell culture , hepatocyte growth factor , hek 293 cells , mesenchymal stem cell , hepatocyte , coagulation , transduction (biophysics) , recombinant dna , cell , immortalised cell line , transfection , stem cell , microbiology and biotechnology , biology , chemistry , viral vector , gene , biochemistry , in vitro , medicine , genetics , receptor
Human mesenchymal stem cells (hMSCs) have the potential to differentiate into hepatocyte-like cells, indicating that these cells may be the new target cell of interest to produce biopharmaceuticals. Our group recently established a hMSC-derived immortalized hepatocyte-like cell line (imHC) that demonstrates several liver-specific phenotypes. However, the ability of imHC to produce coagulation factors has not been characterized. Here, we examined the potential for imHC as a source of coagulation protein production by investigating the ability of imHC to produce human factor VII (FVII) using a lentiviral transduction system. Our results showed that imHC secreted a low amount of FVII (~22 ng/mL) into culture supernatant. Moreover, FVII from the transduced imHC (0.11 ± 0.005 IU/mL) demonstrated a similar coagulant activity compared with FVII from transduced HEK293T cells (0.12 ± 0.004 IU/mL) as determined by chromogenic assay. We demonstrate for the first time, to the best of our knowledge, that imHC produced FVII, albeit at a low level, indicating the unique characteristic of hepatocytes. Our study suggests the possibility of using imHC for the production of coagulation proteins.