Open AccessNormal B cell development and Pax5 expression in Thy28/ThyN1-deficient mice
Author(s) -
Fusako Kitaura,
Miyuki Yuno,
Toshitsugu Fujita,
Shigeharu Wakana,
Junji Ueda,
Kazuya Yamagata,
Hodaka Fujii
Publication year - 2019
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0220199
Subject(s) - pax5 , transcription factor , biology , b cell , microbiology and biotechnology , cell culture , gene , gene expression , cell , regulation of gene expression , genetics , antibody
Thy28, also known as ThyN1, is a highly conserved nuclear protein. We previously showed that in a chicken mature B cell line, Thy28 binds to the promoter of the gene encoding Pax5 , a transcription factor essential for B cell development, and positively regulates its expression. Here, we generated a Thy28 -deficient mouse line to analyze its potential role in B cell development in mice. Thy28 -deficient mice showed normal development of B cells, and the expression of Pax5 was comparable between wild-type and Thy28 -deficient primary B cells. Thus, species-specific mechanisms regulate Pax5 expression and B cell development.