Open AccessEffect of pulsed light on postharvest disease control-related metabolomic variation in melon (Cucumis melo) artificially inoculated with Fusarium pallidoroseum
Author(s) -
Francisco Oiram Filho,
Ebenézer de Oliveira Silva,
Mônica Maria de Almeida Lopes,
Paulo Riceli Vasconselos Ribeiro,
Andréia Hansen Oster,
Jhonyson Arruda Carvalho Guedes,
Dávila Zampieri,
Patrícia do Nascimento Bordallo,
Guilherme Julião Zocolo
Publication year - 2020
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0220097
Subject(s) - postharvest , cucumis , melon , fungicide , biology , fusarium , horticulture , metabolomics , chemistry , food science , botany , bioinformatics
Pulsed light, as a postharvest technology, is an alternative to traditional fungicides, and can be used on a wide variety of fruit and vegetables for sanitization or pathogen control. In addition to these applications, other effects also are detected in vegetal cells, including changes in metabolism and secondary metabolite production, which directly affect disease control response mechanisms. This study aimed to evaluate pulsed ultraviolet light in controlling postharvest rot, caused by Fusarium pallidoroseum in ‘Spanish’ melon, in natura , and its implications in disease control as a function of metabolomic variation to fungicidal or fungistatic effects. The dose of pulsed light (PL) that inhibited F . pallidoroseum growth in melons ( Cucumis melo var. Spanish) was 9 KJ m –2 . Ultra-performance liquid chromatography (UPLC) coupled to a quadrupole-time-of-flight (QTOF) mass analyzer identified 12 compounds based on tandem mass spectrometry (MS/MS) fragmentation patterns. Chemometric analysis by Principal Components Analysis (PCA) and Orthogonal Partial Least Squared Discriminant Analysis (OPLS-DA) and corresponding S-Plot were used to evaluate the changes in fruit metabolism. PL technology provided protection against postharvest disease in melons, directly inhibiting the growth of F . pallidoroseum through the upregulation of specific fruit biomarkers such as pipecolic acid ( 11 ), saponarin ( 7 ), and orientin ( 3 ), which acted as major markers for the defense system against pathogens. PL can thus be proposed as a postharvest technology to prevent chemical fungicides and may be applied to reduce the decay of melon quality during its export and storage.