Open AccessComparison of iron-reduced and iron-supplemented semisynthetic diets in T cell transfer colitis
Author(s) -
Anamarija Markota,
Rebecca Metzger,
Alexander F. Heiseke,
Lisa Jandl,
Ezgi Dursun,
Katharina Eisenächer,
Wolfgang Reindl,
Dirk Haller,
Anne Krug
Publication year - 2019
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0218332
Subject(s) - colitis , inflammation , ferrous , hemin , inflammatory bowel disease , chemistry , proinflammatory cytokine , iron deficiency , ferritin , immunology , dietary iron , iron binding proteins , medicine , biochemistry , anemia , disease , heme , enzyme , organic chemistry , gene
Clinical observations in inflammatory bowel disease patients and experimental studies in rodents suggest that iron in the intestinal lumen derived from iron-rich food or oral iron supplementation could exacerbate inflammation and that iron depletion from the diet could be protective. To test the hypothesis that dietary iron reduction is protective against colitis development, the impact of iron reduction in the diet below 10 mg/kg on the course of CD4+ CD62L+ T cell transfer colitis was investigated in adult C57BL/6 mice. Weight loss as well as clinical and histological signs of inflammation were comparable between mice pretreated with semisynthetic diets with either < 10mg/kg iron content or supplemented with 180 mg/kg iron in the form of ferrous sulfate or hemin. Accumulation and activation of Ly6C high monocytes, changes in dendritic cell subset composition and induction of proinflammatory Th1/Th17 cells in the inflamed colon were not affected by the iron content of the diets. Thus, dietary iron reduction did not protect adult mice against severe intestinal inflammation in T cell transfer induced colitis.