Open AccessAnalysis of bacteria associated with honeys of different geographical and botanical origin using two different identification approaches: MALDI-TOF MS and 16S rDNA PCR technique
Author(s) -
Paweł Pomastowski,
Michał Złoch,
A. Rodzik,
Magdalena Ligor,
Markus Kostrzewa,
Bogusław Buszewski
Publication year - 2019
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0217078
Subject(s) - bacillus cereus , biology , 16s ribosomal rna , micrococcus , bacteria , bacillus (shape) , mycology , microbiology and biotechnology , bacillus subtilis , cereus , botany , genetics
In the presented work identification of microorganisms isolated from various types of honeys was performed. Martix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and 16S rDNA sequencing were applied to study environmental bacteria strains.With both approches, problematic spore-forming Bacillus spp , but also Staphylococcus spp ., Lysinibacillus spp ., Micrococcus spp . and Brevibacillus spp were identified. However, application of spectrometric technique allows for an unambiguous distinction between species/species groups e.g. B . subtilis or B . cereus groups. MALDI TOF MS and 16S rDNA sequencing allow for construction of phyloproteomic and phylogenetic trees of identified bacterial species. Furthermore, the correlation beetween physicochemical properties, geographical and botanical origin and the presence bacterial species in honey samples were investigated.