Open AccessVasculogenically conditioned peripheral blood mononuclear cells inhibit mouse immune response to induced pluripotent stem cell-derived allogeneic cardiac grafts
Author(s) -
Noriyuki Koibuchi,
Shigeru Miyagawa,
Satsuki Fukushima,
Takuji Kawamura,
Akira Kawamura,
Shohei Yoshida,
Yuki Nakamura,
Akira Harada,
Haruchika Masuda,
Köichi Toda,
Takayuki Asahara,
Yoshiki Sawa
Publication year - 2019
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0217076
Subject(s) - peripheral blood mononuclear cell , induced pluripotent stem cell , immunosuppression , immune system , transplantation , immunology , medicine , stem cell , cell therapy , biology , in vitro , microbiology and biotechnology , embryonic stem cell , biochemistry , gene
Allogeneic transplantation of induced pluripotent stem cell (iPSC)-derived cardiomyocytes is apromising treatment for cardiac diseases, although immune rejection by the recipient poses a concern. In this study, we aimed to investigate whether concomitant transplantation of vasculogenically conditioned peripheral blood mononuclear cells, which are otherwise immunosuppressive, may enhance graft survival. Luciferase-transduced, iPSC-derived cardiomyocytes from C57BL/6 mice were transplanted to the dorsal subcutaneous space of syngeneic C57BL/6 mice (n = 19), allogeneic Balb/c mice treated with (n = 20) or without (n = 20) immunosuppressants, and those injected with vasculogenically conditioned peripheral blood mononuclear cells (n = 20). Although graft survival, assessed by bioluminescence, was comparable among the groups initially, it improved significantly at days 7 and 10 in allogeneic transplanted mice treated with vasculogenically conditioned peripheral blood mononuclear cells than in others ( P < 0.01). Our results proved that cell-based immunosuppression may boost clinical outcomes from allogeneic cell therapy.