Open AccessAssessment of TCF7L2 expression after bariatric surgery
Author(s) -
Carlos Eduardo Soares Macedo,
Guilherme da Conti,
Andriu dos Santos Catena,
Danyelly Bruneska,
Michelle Melgarejo da Rosa,
Clarissa Guedes Noronha,
Fernando Santa Cruz,
Álvaro Antônio Bandeira Ferraz
Publication year - 2019
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0216627
Subject(s) - medicine , tcf7l2 , body mass index , diabetes mellitus , prospective cohort study , type 2 diabetes mellitus , correlation , gastroenterology , surgery , endocrinology , biology , genotype , biochemistry , geometry , mathematics , gene , single nucleotide polymorphism
Objective To assess the influence of bariatric surgery on transcription factor 7-like 2 ( TCF7L2 ) expression and its association with body mass index (BMI) and Type 2 diabetes mellitus (T2DM). Methods Prospective study performed between 2016 and 2018, where 26 obese patients undergoing bariatric surgery were divided into two subgroups: diabetics and non-diabetics. The RNAs were extracted from peripheral blood samples that were obtained from each patient in two different moments: before surgery and after 12 months of follow-up. The relative expression of TCF7L2 was determined according to the delta-Ct method. Results The linear regression model of BMI x delta-Ct showed a positive correlation (p = 0.037). In the subgroups, an inversely proportional relationship was found between delta-Ct and BMI in the diabetic group and a directly proportional relationship in the non-diabetic group (p>0.05 in both). In the postoperative period, the regression model was similar to the preoperative, except when analyzing the subgroups, where diabetic patients showed a directly proportional relationship (p>0.05). The relative expression of TCF7L2 showed an average of 1.16 ± 0.91, CI-95% 0.79–1.53. There was an increase in relative expression of 48% in the non-diabetic group (p = 0.021), and a decrease of 27% in the T2DM group (p>0.05) in the postoperative. There was a positive correlation between a greater decrease in BMI and increased relative expression (p = 0.027). Conclusion Our results showed that generally, the TCF7L2 expression increase with a decrease in BMI, however, for patients with T2DM, it exhibits an inverse pattern, which is normalized one year after bariatric surgery.