Open AccessAbundance and diversity of nitrogen-removing microorganisms in the UASB-anammox reactor
Author(s) -
Rui Chen,
Junqin Yao,
Nuerla Ailijiang,
Rui-Sang Liu,
Lei Fang,
Yinguang Chen
Publication year - 2019
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0215615
Subject(s) - anammox , nitrosomonas , microorganism , denitrifying bacteria , archaea , candidatus , environmental chemistry , biology , nitrosomonas europaea , bacteria , anoxic waters , microbiology and biotechnology , chemistry , nitrogen , ecology , denitrification , nitrite , nitrate , 16s ribosomal rna , genetics , organic chemistry
Anaerobic ammonium oxidation is considered to be the most economical and low-energy biological nitrogen removal process. So far, anammox bacteria have not yet been purified from cultures. Some nitrogen-removing microorganisms cooperate to perform the anammox process. The objective of this research was to analyze the abundance and diversity of nitrogen-removing microorganisms in an anammox reactor started up with bulking sludge at room temperature. In this study, the ammonia-oxidizing archaea phylum Crenarchaeota was enriched from 9.2 to 53.0%. Nitrosomonas , Nitrosococcus , and Nitrosospira , which are ammonia-oxidizing bacteria, increased from 3.2, 1.7, and 0.1% to 12.8, 20.4, and 3.3%, respectively. Ca . Brocadia , Ca . Kuenenia , and Ca . Scalindua , which are anammox bacteria, were detected in the seeding sludge, accounting for 77.1, 11.5, and 10.6%. After cultivation, the dominant genus changed to Ca . Kuenenia , accounting for 82.0%. Nitrospirae , nitrite oxidation bacteria, decreased from 2.2 to 0.1%, while denitrifying genera decreased from 12.9 to 2.1%. The results of this study contribute to the understanding of nitrogen-removing microorganisms in an anammox reactor, thereby facilitating the improvement of such reactors. However, the physiological and metabolic functions of the ammonia-oxidizing archaea community in the anammox reactor need to be investigated in further studies.