Open AccessA discriminatory test for the wheat B and G genomes reveals misclassified accessions of Triticum timopheevii and Triticum turgidum
Author(s) -
Beata I. Czajkowska,
Hugo R. Oliveira,
Terence A. Brown
Publication year - 2019
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0215175
Subject(s) - triticum turgidum , biology , genetics , genome , polymerase chain reaction , gene
The tetraploid wheat species Triticum turgidum and Triticum timopheevii are morphologically similar, and misidentification of material collected from the wild is possible. We compared published sequences for the Ppd-A1 , Ppd-B1 and Ppd-G1 genes from multiple accessions of T . turgidum and T . timopheevii and devised a set of four polymerase chain reactions (PCRs), two specific for Ppd-B1 and two for Ppd-G1 . We used these PCRs with 51 accessions of T . timopheevii and 20 of T . turgidum . Sixty of these accessions gave PCR products consistent with their taxon identifications, but the other eleven accessions gave anomalous results: ten accessions that were classified as T . turgidum were identified as T . timopheevii by the PCRs, and one T . timopheevii accession was typed as T . turgidum . We believe that these anomalies are not due to errors in the PCR tests because the results agree with a more comprehensive analysis of genome-wide single nucleotide polymorphisms, which similarly suggest that these eleven accessions have been misclassified. Our results therefore show that the accepted morphological tests for discrimination between T . turgidum and T . timopheevii might not be entirely robust, but that species identification can be made cheaply and quickly by PCRs directed at the Ppd-1 gene.