Open AccessMicrobial metabolite deoxycholic acid shapes microbiota against Campylobacter jejuni chicken colonization
Author(s) -
Bilal Alrubaye,
Mussie Abraha,
Ayidh Almansour,
Mohit Bansal,
Hong Wang,
Young Min Kwon,
Yan Huang,
Billy M. Hargis,
Xiaowei Sun
Publication year - 2019
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0214705
Subject(s) - campylobacter jejuni , microbiology and biotechnology , colonization , biology , campylobacteriosis , deoxycholic acid , campylobacter , glycocholic acid , colonisation resistance , bacteria , bile acid , cholic acid , biochemistry , genetics
Despite reducing the prevalent foodborne pathogen Campylobacter jejuni in chickens decreases campylobacteriosis, few effective approaches are available. The aim of this study was to use microbial metabolic product bile acids to reduce C . jejuni chicken colonization. Broiler chicks were fed with deoxycholic acid (DCA), lithocholic acid (LCA), or ursodeoxycholic acid (UDCA). The birds were also transplanted with DCA modulated anaerobes (DCA-Anaero) or aerobes (DCA-Aero). The birds were infected with human clinical isolate C . jejuni 81–176 or chicken isolate C . jejuni AR101. Notably, C . jejuni 81–176 was readily colonized intestinal tract at d16 and reached an almost plateau at d21. Remarkably, DCA excluded C . jejuni cecal colonization below the limit of detection at 16 and 28 days of age. Neither chicken ages of infection nor LCA or UDCA altered C . jejuni AR101 chicken colonization level, while DCA reduced 91% of the bacterium in chickens at d28. Notably, DCA diet reduced phylum Firmicutes but increased Bacteroidetes compared to infected control birds. Importantly, DCA-Anaero attenuated 93% of C . jejuni colonization at d28 compared to control infected birds. In conclusion, DCA shapes microbiota composition against C . jejuni colonization in chickens, suggesting a bidirectional interaction between microbiota and microbial metabolites.