Open AccessIntensityCheck – The light measuring app for microscope performance checks and consistent fluorescence imaging
Author(s) -
Dirk Dormann
Publication year - 2019
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0214659
Subject(s) - microscope , confocal , stereo microscope , microscopy , optics , materials science , fluorescence microscope , sample (material) , fluorescence , computer science , confocal microscopy , fluorescence lifetime imaging microscopy , biomedical engineering , physics , medicine , thermodynamics
Quantitative fluorescence imaging is an essential tool in biomedical research. It requires consistent and repeatable conditions such as constant sample illumination. Even on a confocal microscope this can usually only be achieved by using an external laser power meter. By combining low-cost wireless Arduino based light sensors with an easy to use Android smartphone app we provide microscope users with a simple but powerful tool to maintain sample illumination for quantitative imaging, for tracking the intensity, stability and alignment of the light sources and for comparing microscope performance.