Open AccessMolecular cytogenetic characterization of repetitive sequences comprising centromeric heterochromatin in three Anseriformes species
Author(s) -
Yoshinobu Uno,
Chizuko Nishida,
Ayano Hata,
Satoshi Ishishita,
Yoichi Matsuda
Publication year - 2019
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0214028
Subject(s) - biology , anseriformes , heterochromatin , galliformes , genetics , microchromosome , haeiii , evolutionary biology , restriction fragment length polymorphism , zoology , chromatin , chromosome , gene , karyotype , polymerase chain reaction
The highly repetitive DNA sequence of centromeric heterochromatin is an effective molecular cytogenetic marker for investigating genomic compartmentalization between macrochromosomes and microchromosomes in birds. We isolated four repetitive sequence families of centromeric heterochromatin from three Anseriformes species, viz., domestic duck ( Anas platyrhynchos , APL), bean goose ( Anser fabalis , AFA), and whooper swan ( Cygnus cygnus , CCY), and characterized the sequences by molecular cytogenetic approach. The 190-bp APL- Hae III and 101-bp AFA- Hinf I-S sequences were localized in almost all chromosomes of A . platyrhynchos and A . fabalis , respectively. However, the 192-bp AFA- Hinf I-L and 290-bp CCY- Apa I sequences were distributed in almost all microchromosomes of A . fabalis and in approximately 10 microchromosomes of C . cygnus , respectively. APL- Hae III, AFA- Hinf I-L, and CCY- Apa I showed partial sequence homology with the chicken nuclear-membrane-associated (CNM) repeat families, which were localized primarily to the centromeric regions of microchromosomes in Galliformes, suggesting that ancestral sequences of the CNM repeat families are observed in the common ancestors of Anseriformes and Galliformes. These results collectively provide the possibility that homogenization of centromeric heterochromatin occurred between microchromosomes in Anseriformes and Galliformes; however, homogenization between macrochromosomes and microchromosomes also occurred in some centromeric repetitive sequences.