Open AccessMining of Indian wheat germplasm collection for adult plant resistance to leaf rust
Author(s) -
Sundeep Kumar,
B. S. Phogat,
V. K. Vikas,
Amit Kumar Sharma,
M. S. Saharan,
Amit Kumar Singh,
Jyoti Kumari,
Rakesh Singh,
Sherry Rachel Jacob,
Gyanendra Singh,
M. Sivasamy,
P. Jayaprakash,
Madhu Meeta,
J. P. Jaiswal,
Deep Shikha,
B. K. Honrao,
I. K. Kalappanavar,
Preeti Mishra,
Shivendra Singh,
S. S. Vaish,
V. A. Solanki
Publication year - 2019
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0213468
Subject(s) - germplasm , biology , rust (programming language) , cultivar , agronomy , plant disease resistance , gene , horticulture , botany , genetics , computer science , programming language
Leaf rust ( Puccinia triticina Eriks.) is a fungal disease of wheat ( Triticum spp.), which causes considerable yield loss. Adult plant resistance (APR) is one of the most sustainable approaches to control leaf rust. In this study, field-testing was carried out across ten different locations, followed by molecular screening, to detect the presence of APR genes, Lr34 +, Lr46 +, Lr67 + and Lr68 in Indian wheat germplasm. In field screening, 190 wheat accessions were selected from 6,319 accessions based on leaf tip necrosis (LTN), disease severity and the average coefficient of infection. Molecular screening revealed that 73% of the accessions possessed known APR genes either as single or as a combination of two or three genes. The occurrence of increased LTN intensity, decreased leaf rust severity and greater expression of APR genes were more in relatively cooler locations. In 52 lines, although the presence of the APR genes was not detected, it still displayed high levels of resistance. Furthermore, 49 accessions possessing either two or three APR genes were evaluated for stability across locations for grain yield. It emerged that eight accessions had wider adaptability. Resistance based on APR genes, in the background of high yielding cultivars, is expected to provide a high level of race non-specific resistance, which is durable.