Open AccessMolecular analysis of small tissue samples obtained via transbronchial lung biopsy using radial probe endobronchial ultrasound
Author(s) -
Insu Kim,
Jung Seop Eom,
Ah rong Kim,
Chang-Hun Lee,
Geewon Lee,
Eun Jung Jo,
MiHyun Kim,
Jeongha Mok,
Kwangha Lee,
Ki Uk Kim,
Hye Kyung Park,
Min Ki Lee
Publication year - 2019
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0212672
Subject(s) - lung cancer , anaplastic lymphoma kinase , medicine , concordance , epidermal growth factor receptor , biopsy , lung , pathology , ultrasound , chromosomal translocation , radiology , cancer , biology , gene , biochemistry , malignant pleural effusion
Background Radial probe endobronchial ultrasound using a guide sheath (EBUS-GS) is used to diagnose peripheral lung cancer. The aim was to identify the accuracy of molecular analysis that were performed with EBUS-GS specimens in patients with non-small cell lung cancer (NSCLC). Method From December 2015 to September 2017, we retrospectively studied 91 patients with peripheral NSCLC who underwent surgery after EBUS-GS. Epidermal growth factor receptor ( EGFR ) mutational and anaplastic lymphoma kinase ( ALK ) translocation status obtained from surgical specimens served as the references. Results Compared to the reference data, EGFR mutational testing of EBUS-GS specimens was in 97% agreement, and the κ coefficient was 0.931 (P< 0.001). In addition, on ALK translocation testing, the results of all 91 patients were in agreement with the reference data (concordance rate of 100%, κ coefficient 1.000; P< 0.001). Conclusion We found that EBUS-GS could be used for molecular diagnosis, such as EGFR mutational and ALK translocation status, in patients with peripheral NSCLC.