Open AccessProtein features for assembly of the RNA editing helicase 2 subcomplex (REH2C) in Trypanosome holo-editosomes
Author(s) -
Vijoy Kumar,
Pawan Kumar Doharey,
Shelly Gulati,
Joshua Meehan,
Mary Grace Martinez,
Karrisa Hughes,
Blaine H. M. Mooers,
Jorge CruzReyes
Publication year - 2019
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0211525
Subject(s) - rna editing , biology , ribonucleoprotein , helicase , rna , rna helicase a , zinc finger , rna binding protein , microbiology and biotechnology , guide rna , genetics , computational biology , genome editing , transcription factor , gene , crispr
Uridylate insertion/deletion RNA editing in Trypanosoma brucei is a complex system that is not found in humans, so there is interest in targeting this system for drug development. This system uses hundreds of small non-coding guide RNAs (gRNAs) to modify the mitochondrial mRNA transcriptome. This process occurs in holo-editosomes that assemble several macromolecular trans factors around mRNA including the RNA-free RNA editing core complex (RECC) and auxiliary ribonucleoprotein (RNP) complexes. Yet, the regulatory mechanisms of editing remain obscure. The enzymatic accessory RNP complex, termed the REH2C, includes mRNA substrates and products, the multi-domain 240 kDa RNA Editing Helicase 2 (REH2) and an intriguing 8-zinc finger protein termed REH2-Associated Factor 1 ( H2 F1). Both of these proteins are essential in editing. REH2 is a member of the DExH/RHA subfamily of RNA helicases with a conserved C-terminus that includes a regulatory OB-fold domain. In trypanosomes, H2 F1 recruits REH2 to the editing apparatus, and H2 F1 downregulation causes REH2 fragmentation. Our systematic mutagenesis dissected determinants in REH2 and H2 F1 for the assembly of REH2C, the stability of REH2, and the RNA-mediated association of REH2C with other editing trans factors. We identified functional OB-fold amino acids in eukaryotic DExH/RHA helicases that are conserved in REH2 and that impact the assembly and interactions of REH2C. H2 F1 upregulation stabilized REH2 in vivo . Mutation of the core cysteines or basic amino acids in individual zinc fingers affected the stabilizing property of H2 F1 but not its interactions with other examined editing components. This result suggests that most, if not all, fingers may contribute to REH2 stabilization. Finally, a recombinant REH2 (240 kDa) established that the full-length protein is a bona fide RNA helicase with ATP-dependent unwinding activity. REH2 is the only DExH/RHA-type helicase in kinetoplastid holo-editosomes.