Open AccessPKC regulates the production of fibroblast growth factor 23 (FGF23)
Author(s) -
Ludmilla Bär,
Philipp Hase,
Michael Föller
Publication year - 2019
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0211309
Subject(s) - protein kinase c , diacylglycerol kinase , calphostin c , activator (genetics) , microbiology and biotechnology , fibroblast growth factor 23 , chemistry , signal transduction , fibroblast growth factor , osteoblast , phorbol , tyrosine kinase , biochemistry , biology , receptor , parathyroid hormone , calcium , organic chemistry , in vitro
Serine/threonine protein kinase C (PKC) is activated by diacylglycerol that is released from membrane lipids by phospholipase C in response to activation of G protein-coupled receptors or receptor tyrosine kinases. PKC isoforms are particularly relevant for proliferation and differentiation of cells including osteoblasts. Osteoblasts/osteocytes produce fibroblast growth factor 23 (FGF23), a hormone regulating renal phosphate and vitamin D handling. PKC activates NFκB, a transcription factor complex controlling FGF23 expression. Here, we analyzed the impact of PKC on FGF23 synthesis. Fgf23 expression was analyzed by qRT-PCR in UMR106 osteoblast-like cells and in IDG-SW3 osteocytes, and FGF23 protein was measured by ELISA. Phorbol ester 12-O-tetradecanoylphorbol-13-acetate (PMA), a PKC activator, up-regulated FGF23 production. In contrast, PKC inhibitors calphostin C, Gö6976, sotrastaurin and ruboxistaurin suppressed FGF23 formation. NFκB inhibitor withaferin A abolished the stimulatory effect of PMA on Fgf23 . PKC is a powerful regulator of FGF23 synthesis, an effect which is at least partly mediated by NFκB.