Open AccessOptimization of Ralstonia solanacearum cell growth using a central composite rotational design for the P(3HB) production: Effect of agitation and aeration
Author(s) -
Mariane Igansi Alves,
Karine Laste Macagnan,
Camila Rios Piecha,
Matheus Marques Torres,
Izadora Almeida Perez,
Sônia Maria Kesserlingh,
Rosane da Silva Rodrigues,
Patrícia Diaz de Oliveira,
Angelita da Silveira Moreira
Publication year - 2019
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0211211
Subject(s) - polyhydroxyalkanoates , ralstonia solanacearum , aeration , bioreactor , fermentation , ralstonia , metabolism , food science , chemistry , materials science , biochemistry , microbiology and biotechnology , biology , bacteria , organic chemistry , enzyme , genetics , pathogen
The intracellular accumulation of polyhydroxyalkanoates (PHAs) normally occurs after cell growth, during the second fermentation stage and under nutrient-limited conditions in the presence of a carbon excess. However, some microorganisms are able to accumulate PHAs as poly(3-hydroxybutyrate) [P(3HB)] during the first fermentation stage, the cell growth phase, without nutrient limitation, once they have been reported to utilize type II metabolism during the polymer accumulation phase. This study evaluated the effect of aeration and agitation on cell growth and P(3HB) accumulation in Ralstonia solanacearum RS, performed in a bioreactor for 24h at 32°C. A 2 2 central composite rotational design (CCRD) was used, with agitation (150 to 250 rpm) and aeration (0.3 to 1 vvm) as independent variables and optical density (OD 600nm ), dry cell weight (DCW), and P(3HB) yield as dependent variables. A significant polymer accumulation, until 70% of P(3HB), was observed, proving that R . solanacearum RS exhibited metabolism type II, regardless of the aeration process. The best results were obtained for 1 vvm and 250 rpm (+1, +1), with values of OD 600nm (18.04) and DCW (4.82 g.L -1 ).