Open AccessVirus load and clinical features during the acute phase of Chikungunya infection in children
Author(s) -
B. Siva Raghavendhar,
Ashok Kumar Patel,
Sushil Kumar Kabra,
Rakesh Lodha,
Vinod H. Ratageri,
Pratima Ray
Publication year - 2019
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0211036
Subject(s) - myalgia , chikungunya , virus , medicine , viral load , virology , immunology
Background Chikungunya virus (CHIKV) infection is a long known mosquito-borne disease that is associated with severe morbidity, characterized by fever, headache, rashes, joint pain, and myalgia. It is believed that virus load has relation with severity of clinical features. Objectives We performed this study to assess the relationship between virus load and clinical features in children during the acute phase of CHIKV infection, in order to draw insights for better-informed treatment. Study design Between June 1, 2009, and May 31, 2010, 338 patients with fever and susceptive to CHIKV during first 4 days of illness were prospectively enrolled from Karnataka Institute of Medical Sciences, Hubli in our hospital based cross sectional observational study. Sybr green quantitative reverse transcription polymerase chain reaction was performed to estimate the virus load. Results Quantitative RT-PCR was positive for CHIKV in 54 patients. The median copy number of CHIKV was 1.3x 10 8 copies/ml (1.7x10 5 -9.9x10 9 copies/ml). Among the observed clinical features, a statistically significant difference in log mean virus load was found between patients with and without myalgia (log mean 7.50 vs 8.34, P = 0.01). Conclusion Patients with myalgia had lower virus load and those without myalgia had a higher virus load.