Open AccessExpression of CD25 fluctuates in the leukemia-initiating cell population of CD25-positive AML
Author(s) -
Yuki Kageyama,
Hiroshi Miwa,
Rino Arakawa,
Isao Tawara,
Kohshi Ohishi,
Masahiro Masuya,
Kazunori Nakase,
Naoyuki Katayama
Publication year - 2018
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0209295
Subject(s) - il 2 receptor , myeloid leukemia , population , leukemia , cd34 , cancer research , acute myeloblastic leukemia , transplantation , biology , immunology , medicine , stem cell , t cell , microbiology and biotechnology , immune system , environmental health
CD25 is expressed on leukemic cells in 10–20% cases of acute myeloid leukemia (AML), and its expression is associated with poor prognosis. We reevaluated the relationship between CD25 expression and the leukemia-initiating cell (LIC) properties of AML using a patient-derived xenograft model. We divided lineage marker-negative (Lin – ) CD34 + CD38 – or Lin – CD34 + cells from CD25-positive AML into CD25-positive and -negative populations, and then transplanted each population into NOD.Cg- Prkdc scid Il2rg tm1Wjl /Sz mice. Leukemic engraftment was observed with both CD25-positive and -negative populations from three of nine CD25-positive AML patients. In two of those three patients, CD25-positive and -negative Lin – CD34 + cells engrafted at the primary transplantation led to leukemic engraftment at the secondary transplantation, in which engrafted cells contained both CD25-positive and -negative Lin – CD34 + AML cells. In an in vitro culture system, expression of CD25 was considerably induced in the CD25-negative population of Lin – CD34 + cells from two cases of CD25-positive AML. In one case, CD25-positive Lin – CD34 + cells gave rise to CD25-negative as well as -positive CD34 + cells. These observations suggest that there exist CD25-positive and -negative populations that can reconstitute CD25-positive AML in a patient-derived xenograft model, and that CD25 expression fluctuates in the LICs of AML.