Open AccessComprehensive analysis of miRNA and protein profiles within exosomes derived from canine lymphoid tumour cell lines
Author(s) -
Hiroshi Asada,
Hirotaka Tomiyasu,
Takao Uchikai,
Genki Ishihara,
Yuko GotoKoshino,
Koichi Ohno,
Hajime Tsujimoto
Publication year - 2019
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0208567
Subject(s) - microvesicles , microrna , biology , cell culture , cell , microbiology and biotechnology , immune system , crosstalk , extracellular vesicle , cancer research , immunology , genetics , gene , physics , optics
Exosomes are small extracellular vesicles released from almost all cell types, which play roles in cell-cell communication. Recent studies have suggested that microenvironmental crosstalk mediated by exosomes is an important factor in the escape of tumour cells from the anti-tumour immune system in human haematopoietic malignancies. Here, we conducted comprehensive analysis of the miRNA and protein profiles within the exosomes released from four canine lymphoid tumour cell lines as a model of human lymphoid tumours. The results showed that the major miRNAs and proteins extracted from the exosomes were similar among the four cell lines. However, the miRNA profiles differed among the exosomes of each cell line, which corresponded to the expression patterns of the parent cells. In the comparison of the amounts of miRNAs and proteins among the cell lines, those of three miRNAs (miR-151, miR-8908a-3p, and miR-486) and CD82 protein differed between exosomes derived from vincristine-sensitive and resistant cell lines. Further investigations are needed to elucidate the biological functions of the exosomal contents in the microenvironmental crosstalk of lymphoid tumours.