Open AccessOverexpression of ScMYBAS1 alternative splicing transcripts differentially impacts biomass accumulation and drought tolerance in rice transgenic plants
Author(s) -
Rafael Fávero Peixoto-Júnior,
Larissa Mara de Andrade,
Mónica Brito,
Paula Macedo Nóbile,
Alexandre Palma Boer Martins,
Samira Domingues Carlin,
Rafael Vasconcelos Ribeiro,
Maria Helena S. Goldman,
João Felipe Nebó Carlos de Oliveira,
Antônio Figueira,
Silvana Creste
Publication year - 2018
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0207534
Subject(s) - drought tolerance , biology , genetically modified rice , genetically modified crops , myb , biomass (ecology) , transgene , agronomy , abiotic stress , botany , gene , gene expression , genetics
Drought is the most significant environmental stress for agricultural production worldwide, and tremendous efforts have been made to improve crop yield under the increasing water scarcity. Transcription factors are major players in the regulation of water stress-related genes in plants. Recently, different MYB transcription factors were characterized for their involvement in drought response. A sugarcane R2R3-MYB gene ( ScMYBAS1 ) and its four alternative forms of transcript ( ScMYAS1-2 , ScMYBAS1-3 , ScMYBAS1-4 and ScMYBAS1-5 ) were identified in this study. The subcellular localization, in Nicotiniana benthamiana , of the TFs fused in frame with GFP revealed that ScMYBAS1-2-GFP and ScMYBAS1-3-GFP were observed in the nucleus. The overexpression of ScMYBAS1-2 and ScMYBAS1-3 spliced transcripts in rice promoted change in plant growth under both well-watered and drought conditions. The ScMYBAS1-2 and ScMYBAS1-3 transgenic lines revealed a higher relative water content (RWC) compared to the wild type before maximum stress under drought conditions. The ScMYBAS1-2 transgenic lines showed a reduction in biomass (total dry weight). Conversely, ScMYBAS1-3 showed an increased biomass (total dry weight) relative to the wild-type. The overexpression of ScMYBAS1-3 in rice transgenic lines showed involvement with drought tolerance and biomass and, for this reason, was considered a good target for plant transformation, particularly for use in developing genotypes with drought tolerance and biomass accumulation.