Open AccessEffects of a single transient transfection of Ten-eleven translocation 1 catalytic domain on hepatocellular carcinoma
Author(s) -
Yuying Liu,
Hui Zhu,
Zhenxue Zhang,
Changchun Tu,
Dongyuan Yao,
Bin Wen,
Ru Jiang,
Xing Li,
Pengfei Yi,
Jiejie Zhan,
Jiaping Hu,
Jingjin Ding,
Liping Jiang,
Fanglin Zhang
Publication year - 2018
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0207139
Subject(s) - transfection , methylation , cancer research , microbiology and biotechnology , hepatocellular carcinoma , dna methylation , biology , chromosomal translocation , chemistry , gene , gene expression , genetics
Tumor suppressor genes (TSGs), including Ten-eleven translocation 1 (TET1), are hypermethylated in hepatocellular carcinoma (HCC). TET1 catalytic domain (TET1-CD) induces genome-wide DNA demethylation to activate TSGs, but so far, anticancer effects of TET1-CD are unclear. Here we showed that after HCC cells were transiently transfected with TET1-CD, the methylation levels of TSGs, namely APC, p16, RASSF1A, SOCS1 and TET1, were distinctly reduced, and their mRNA levels were significantly increased and HCC cells proliferation, migration and invasion were suppressed, but the methylation and mRNA levels of oncogenes, namely C-myc, Bmi1, EMS1, Kpna2 and c-fos, were not significantly change. Strikingly, HCC subcutaneous xenografts in nude mice remained to be significantly repressed even 54 days after transient transfection of TET1-CD. So, transient transfection of TET1-CD may be a great advance in HCC treatment due to its activation of multiple TSGs and persistent anticancer effects.