Open AccessSpread of GES-5 carbapenemase-producing Pseudomonas aeruginosa clinical isolates in Japan due to clonal expansion of ST235
Author(s) -
Tomomi Hishinuma,
Tatsuya Tada,
Kyoko KuwaharaArai,
Norio Yamamoto,
Masayuki Shimojima,
Teruo Kirikae
Publication year - 2018
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0207134
Subject(s) - pseudomonas aeruginosa , biology , microbiology and biotechnology , isolation (microbiology) , outbreak , bacteria , genetics , virology
The first outbreak in Japan of GES-5 carbapenemase-producing Pseudomonas aeruginosa occurred in a long-term care facility in 2014. To assess the spread of GES-5 producing P . aeruginosa clinical isolates in medical settings in Japan, 1,476 carbapenem-resistant P . aeruginosa isolates obtained from 2012 to 2016 were characterized. Of these 1,476 isolates, 104 (7.0%) harbored bla GES-5 . Southern blotting revealed that the bla GES-5 was located on the chromosome. The isolation rates of these GES-5 producers increased significantly every year, from 2.0% (6 of 295) in 2012 to 2.8% (8 of 283) in 2013 to 5.3% (16 of 303) in 2014 to 9.7% (29 of 300) in 2015 to 15.3% (45 of 295) in 2016. Of the 104 GES-5 producers, 102 belonged to clonal complex (CC) 235, including 99 belonging to ST235 and three belonging to ST2233). Whole genome sequence analysis revealed that CC235 P . aeruginosa harboring bla GES-5 spread in a clonal manner. These results indicate that these GES-5 producing CC235 P . aeruginosa clinical isolates have spread in medical settings throughout Japan.