Open AccessCells respond to deletion of CAV1 by increasing synthesis of extracellular matrix
Author(s) -
Carolina Mendoza-Topaz,
Geoffrey M. Nelson,
Gillian Howard,
Sebastian Häfner,
Peter Rademacher,
Manfred Frick,
Benjamin J. Nichols
Publication year - 2018
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0205306
Subject(s) - caveolae , extracellular matrix , microbiology and biotechnology , biology , extracellular , caveolin 1 , cell culture , hek 293 cells , gene expression profiling , gene expression , gene , genetics , signal transduction
A range of cellular functions have been attributed to caveolae, flask-like invaginations of the plasma membrane. Here, we have used RNA-seq to achieve quantitative transcriptional profiling of primary embryonic fibroblasts from caveolin 1 knockout mice ( CAV1-/- MEFs), and thereby to gain hypothesis-free insight into how these cells respond to the absence of caveolae. Components of the extracellular matrix were decisively over-represented within the set of genes displaying altered expression in CAV1-/- MEFs when compared to congenic wild-type controls. This was confirmed biochemically and by imaging for selected examples. Up-regulation of components of the extracellular matrix was also observed in a second cell line, NIH-3T3 cells genome edited to delete CAV1 . Up-regulation of components of the extracellular matrix was detected in vivo by assessing collagen deposition and compliance of CAV1-/- lungs. We discuss the implications of these findings in terms of the cellular function of caveolae.