Open AccessSerology, virulence and molecular characteristics of Vibrio parahaemolyticus isolated from seafood in Zhejiang province
Author(s) -
Xiao Chen,
Qiaoyun Zhu,
Fei Yu,
Zhang Wen,
Ruonan Wang,
Xianfei Ye,
Liu Jin,
Yanchao Liu,
Shufei Li,
Yu Chen
Publication year - 2018
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0204892
Subject(s) - vibrio parahaemolyticus , virulence , serotype , microbiology and biotechnology , biology , pathogen , gene , virology , multilocus sequence typing , strain (injury) , genotype , genetics , bacteria , anatomy
Vibrio parahaemolyticus is a leading foodborne pathogen in southeastern China. In this study, 105 strains of V . parahaemolyticus were isolated from fresh seafood in 2013 and 2014. The serotypes, virulence-associated genes and sequence types (STs) of these strains were analyzed. 26 defined serotypes were identified and 69 strains (65.7%) had untypeable O or K antigen. 8 strains (7.6%) had the virulence-associated gene tdh and no strain carried the trh gene. 45.7% (48/105) of isolates contained all four T3SS1 genes and 50% (4/8) tdh + trh - V . parahaemolyticus isolates lacked at least one of the four tested T3SS2α genes. 105 strains could be categorized into 84 STs and only 3 STs (ST3, ST8, ST675) had appeared in clinical strains. V . parahaemolyticus strains from seafood have more diverse and untypeable serotypes, less virulence-associated genes and more STs than strains from clinical sources.