Open AccessBlood outgrowth endothelial cells (BOECs) as a novel tool for studying adhesion of Plasmodium falciparum-infected erythrocytes
Author(s) -
Gertrude Ecklu-Mensah,
Rebecca W. Olsen,
Anja Bengtsson,
Michael F. Ofori,
Lars Hviid,
Anja T. R. Jensen,
Yvonne Adams
Publication year - 2018
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0204177
Subject(s) - biology , cd31 , peripheral blood mononuclear cell , thrombomodulin , population , microbiology and biotechnology , endothelial stem cell , cell adhesion molecule , cell adhesion , plasmodium falciparum , immunology , adhesion , in vitro , endothelium , platelet , cell , malaria , chemistry , medicine , thrombin , biochemistry , immunohistochemistry , genetics , environmental health , organic chemistry
The lack of suitable animal models for the study of cytoadhesion of P . falciparum- infected erythrocytes (IEs) has necessitated in vitro studies employing a range of cell lines of either human tumour origin (e.g., BeWo and C32 cells) or non-human origin (e.g., CHO cells). Of the human cells available, many were isolated from adults, or derived from a pool of donors (e.g., HBEC-5i). Here we demonstrate, for the first time, the successful isolation of blood outgrowth endothelial cells (BOECs) from frozen stabilates of peripheral blood mononuclear cells obtained from small-volume peripheral blood samples from paediatric malaria patients. BOECs are a sub-population of human endothelial cells, found within the peripheral blood. We demonstrate that these cells express receptors such as Intercellular Adhesion Molecule 1 (ICAM-1/CD54), Endothelial Protein C Receptor (EPCR/CD201), platelet/endothelial cell adhesion molecule 1 (PECAM-1/CD31), Thrombomodulin (CD141), and support adhesion of P . falciparum IEs.