Open AccessMiR-18a regulates myoblasts proliferation by targeting Fgf1
Author(s) -
Chuncheng Liu,
Min Chen,
Meng Wang,
Wen-Hui Pi,
Ning Li,
Qingyong Meng
Publication year - 2018
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0201551
Subject(s) - c2c12 , fgf1 , cell growth , biology , microbiology and biotechnology , untranslated region , microrna , three prime untranslated region , gene , myocyte , cancer research , fibroblast growth factor , messenger rna , genetics , myogenesis , receptor , fibroblast growth factor receptor
MiRNAs play an important role in cell proliferation, apoptosis, and differentiation. MiR-18a is increasingly being recognized as a regulator of cancer pathogenesis. Here, we discovered that miR-18a participates in myoblasts proliferation. Expression of miR-18a was downregulated with the differentiation of C2C12 myoblasts. Overexpression of miR-18a affected the proliferation of C2C12 cells, primary myoblasts and RD cells. MiR-18a influenced the expression of cell cycle-related genes. Using TargetScan 6.2, we found that the 3’ untranslated region (UTR) of the mouse Fgf1 gene contains complementary sequences to miR-18a. Using a siRNA, we confirmed that the reduction in the Fgf1 levels inhibited proliferation of C2C12 cells. Therefore, our results show that miR-18a participates in the regulation of proliferation by partly decreasing the expression of Fgf1.