Open AccessRecovery from an acute systemic and central LPS-inflammation challenge is affected by mouse sex and genetic background
Author(s) -
Gabriela Meneses,
M. Rosetti,
Alejandro Espinosa,
A. Florentino,
M. Bautista,
Georgina Díaz,
Guillermo Olvera,
Brandon Bárcena,
Agnès Fleury,
Laura AdalidPeralta,
Edmundo Lamoyi,
Gladis Fragoso,
Edda Sciutto
Publication year - 2018
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0201375
Subject(s) - microglia , lipopolysaccharide , peripheral , tumor necrosis factor alpha , inflammation , medicine , endocrinology , pathogenesis , hippocampus , systemic inflammation , immunology , interleukin 6 , cytokine
Genetic and sexual factors influence the prevalence and the pathogenesis of many inflammatory disorders. In this study their relevance on the peripheral and central inflammatory status induced by a peripheral injection of lipopolysaccharide (LPS) was evaluated. BALB/c and CD-1 male and female mice were intraperitoneally injected with LPS. Spleens and brains were collected 2 and 72 hours later to study the levels of IL-6, TNF-α and IL-1β. Percentage of microglia and astrocytes was determined in the cortex and hippocampus. Locomotor activity was registered before and during the 72 hours after LPS-treatment. Two hours after LPS-injection, a peripheral increase of the three cytokines was found. In brains, LPS increased TNF-α only in males with higher levels in CD-1 than BALB/c. IL-1β increased only in CD-1 males. IL-6 increased in both strains with lower levels in BALB/c females. Peripheral and central levels of cytokines decline 72 hrs after LPS-treatment whilst a significantly increase of Iba-1 expression was detected. A dramatic drop of the locomotor activity was observed immediately after LPS injection. Our results show that acute systemic administration of LPS leads to peripheral and central increase of pro-inflammatory cytokines and microglia activation, in a strain and sex dependent manner.