Open AccessSpatial regulation of expanded transcription in the Drosophila wing imaginal disc
Author(s) -
Lan Hsin Wang,
Nicholas E. Baker
Publication year - 2018
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0201317
Subject(s) - biology , transcription factor , enhancer , imaginal disc , notch signaling pathway , decapentaplegic , microbiology and biotechnology , hippo signaling pathway , transcription (linguistics) , genetics , zinc finger transcription factor , krüppel , repressor , drosophila melanogaster , zinc finger , signal transduction , gene , linguistics , philosophy
Growth and patterning are coordinated during development to define organ size and shape. The growth, proliferation and differentiation of Drosophila wings are regulated by several conserved signaling pathways. Here, we show that the Salvador-Warts-Hippo (SWH) and Notch pathways converge on an enhancer in the expanded ( ex ) gene, which also responds to levels of the bHLH transcription factor Daughterless (Da). Separate cis-regulatory elements respond to Salvador-Warts-Hippo (SWH) and Notch pathways, to bHLH proteins, and to unidentified factors that repress ex transcription in the wing pouch and in the proneural region at the anterior wing margin. Senseless, a zinc-finger transcription factor acting in proneural regions, had a negative impact on ex transcription in the proneural region, but the transcriptional repressor Hairy had no effect. Our study suggests that a complex pattern of ex transcription results from integration of a uniform SWH signal with multiple other inputs, rather than from a pattern of SWH signaling.