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Genetic ablation of interacting with Spt6 (Iws1) causes early embryonic lethality
Author(s) -
Arturo Orlacchio,
Aaron E. Stark,
Claudia Foray,
Foued Amari,
Tyler Sheetz,
Erika Reese,
Anna Tessari,
Krista La Perle,
Dario Palmieri,
Philip N. Tsichlis,
Vincenzo Coppola
Publication year - 2018
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0201030
Subject(s) - biology , embryonic stem cell , gene expression , microbiology and biotechnology , gene , embryogenesis , lethality , embryo , transcription factor , messenger rna , transcription (linguistics) , rna , rna polymerase ii , genetics , promoter , linguistics , philosophy
IWS1 is an RNA-polymerase II (RNAPII)-associated transcription elongation factor whose biological functions are poorly characterized. To shed some light on the function of this protein at the organismal level, we performed a systematic tissue analysis of its expression and generated Iws1 -deficient mice. A thorough immunohistochemical characterization shows that IWS1 protein is present in the nucleus of all cells in most of the examined tissues, with few notable exceptions. We also report that ablation of Iws1 consistently causes lethality at the pre-implantation stage with high expression of the gene in fertilized oocytes. In summary, we are providing evidence that Iws1 is expressed in all adult organs and it is an essential gene for mouse embryonic development.

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