Open AccessEvaluation of the bag-mediated filtration system as a novel tool for poliovirus environmental surveillance: Results from a comparative field study in Pakistan
Author(s) -
Nicolette A. Zhou,
Christine S. Fagnant-Sperati,
Jeffry H. Shirai,
Salman Sharif,
Syed Sohail Zahoor Zaidi,
Lubna Rehman,
Jaffer Hussain,
Rahim D. Agha,
Shahzad Shaukat,
Masroor Alam,
Adnan Khurshid,
Ghulam Mujtaba,
Muhammed Barjes Salman,
Rana Muhammad Safdar,
Abdirahman Mahamud,
Jamal Ahmed,
Sadaf Khan,
Alexandra L. Kossik,
Nicola K. Beck,
G. Matrajt,
Humayun Asghar,
Ananda S Bandyopadhyay,
David Scott Boyle,
John Scott Meschke
Publication year - 2018
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0200551
Subject(s) - biology , serotype , virology , poliovirus , virus
Poliovirus (PV) environmental surveillance (ES) plays an important role in the global eradication program and is crucial for monitoring silent PV circulation especially as clinical cases decrease. This study compared ES results using the novel bag-mediated filtration system (BMFS) with the current two-phase separation method. From February to November 2016, BMFS and two-phase samples were collected concurrently from twelve sites in Pakistan ( n = 117). Detection was higher in BMFS than two-phase samples for each Sabin-like (SL) PV serotype ( p <0.001) and wild PV type 1 (WPV1) ( p = 0.065). Seventeen sampling events were positive for WPV1, with eight discordant in favor of BMFS and two in favor of two-phase. A vaccine-derived PV type 2 was detected in one BMFS sample but not the matched two-phase. After the removal of SL PV type 2 (SL2) from the oral polio vaccine in April 2016, BMFS samples detected SL2 more frequently than two-phase ( p = 0.016), with the last detection by either method occurring June 12, 2016. More frequent PV detection in BMFS compared to two-phase samples is likely due to the greater effective volume assayed (1620 mL vs. 150 mL). This study demonstrated that the BMFS achieves enhanced ES for all PV serotypes in an endemic country.