Open AccessMiR-221 negatively regulates innate anti-viral response
Author(s) -
Hongqiang Du,
Shuang Cui,
Yunfei Li,
Guang Yang,
Peiyan Wang,
Erol Fikrig,
Fangtian You
Publication year - 2018
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0200385
Subject(s) - innate immune system , gene knockdown , regulator , biology , microrna , microbiology and biotechnology , immune system , negative regulator , immunology , cell culture , signal transduction , gene , genetics
The innate immune system plays a critical role in the initial antiviral response. However, the timing and duration of these responses must be tightly regulated during infection to ensure appropriate immune cell activation and anti-viral defenses. Here we demonstrate that during antiviral response, a negative regulator miR-221 was also induced in an ELF4-dependent manner. We further show that ELF4 promotes miR-221 expression through direct binding to its promoter. Overexpression and knockdown assay show that miR-221 can negatively regulate IFNβ production in time of virus infection. RNA-seq analysis of miR-221 overexpressed cells revealed multiple candidate targets. Taken together, our study identified a novel negative microRNA regulator of innate antiviral response, which is dependent on ELF4.