Open AccessAntimicrobial activity of NO-releasing compounds against periodontal pathogens
Author(s) -
Ji Suk Shim,
Dong Sik Park,
Dong Heon Baek,
Nayansi Jha,
Serk In Park,
Hyoung Jin Yun,
Won Jong Kim,
Jae Jun Ryu
Publication year - 2018
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0199998
Subject(s) - aggregatibacter actinomycetemcomitans , cytotoxicity , antimicrobial , porphyromonas gingivalis , microbiology and biotechnology , chemistry , periodontitis , mtt assay , antibacterial activity , minimum inhibitory concentration , streptococcus mutans , bacteria , in vitro , biochemistry , biology , medicine , dentistry , genetics
This study describes the successful synthesis of nitric oxide (NO)-releasing compounds with biodegradable and injectable properties and demonstrates that the kinetics of NO release vary according to the type of NO donor. The antimicrobial activity of NO-releasing compounds against three common periodontal pathogens, i.e., Aggregatibacter actinomycetemcomitans , Porphyromonas gingivalis , and Actinomyces israelii , was investigated using a susceptibility assay. Human gingival fibroblasts were treated with NO-releasing compounds at the minimum concentrations required for bacterial growth and cytotoxicity was evaluated using the MTT cell proliferation assay. Our results suggest that NO-releasing compounds can be used topically to treat both gram-negative and gram-positive periodontal pathogens. Comparison of the antimicrobial activity and cytotoxicity assay results between the NO-releasing compounds revealed that an NO donor comprising a macromolecule without surface charge, a lower instantaneous NO concentration, and an adequate supply of NO were associated with a strong bactericidal effect and low cytotoxicity. NO-releasing compounds with these properties may be suitable for treatment of periodontitis.